What we do
We are the only breast tissue bank in the UK offering primary cells isolated from human breast tissues. We also provide information and advice to researchers who would like to use primary cells.
Currently, we can offer tissue explants, organoids and purified populations of cells isolated from normal, high-risk, cancer-containing and malignant breast tissue. Some of the cell populations are suitable to be grown in culture and can be used successfully in a range of functional assays. Others are suitable for DNA, RNA or protein extraction. Currently, tumour epithelial cells are not suitable for culture assays.
Note: when providing cells, we can reserve samples of the same batch, so that should you require further vials these will be available.
Cell types available from:
|normal: cosmetic reduction mammoplasty|
|contralateral: tumour in other breast|
|DCIS surround: ≥5cm from DCIS edge|
|tumour: invasive carcinoma|
|adjacent to tumour: ≤2cm from
|tumour surround: ≥5cm from tumour edge.|
Sample types available:
|live explants (typically 10 per vial)|
|mixed cell preparations|
|purified cell types for tissue culture|
|cell pellets from purified cell types|
|DNA, RNA extracts|
|cytoblocks from isolated cell populations.|
We have tissue and cell stocks available, but we are interested in discussing prospective preparations or collections as required. We can provide advice on:
- the choice of cells for your studies
- guidelines for the handling and culturing of each cell type
- details of preferred growth media
- comprehensive protocols for specific applications
- planning your experiments and estimating costs for grant applications
- availability of reserved cell batches.
Please email us at firstname.lastname@example.org with any questions about our service.
- Isolation of pure populations of epithelial and myoepithelial cells from the normal human mammary gland using immunomagnetic separation with Dynabeads. Gomm JJ, et al. Analytical Biochemistry. 1995; 226:91–99.
- Separated human breast epithelial and myoepithelial cells have different growth factor requirements in vitro but can reconstitute normal breast lobuloalveolar structure. Gomm JJ, et al. J Cell Physiol. 1997; 171(1):11–19.
- Novel multicellular organotypic models of normal and malignant breast: tools for dissecting the role of the microenvironment in breast cancer progression. Holliday DL, et al. Breast Cancer Res. 2009; 11(1):R3.
- Intrinsic genetic characteristics determine tumor-modifying capacity of fibroblasts: matrix metalloproteinase-3 5A/5A genotype enhances breast cancer cell invasion. Holliday DL, et al. Breast Cancer Res. 2007; 9(5):R67.
- Primary breast myoepithelial cells exert an invasion-suppressor effect on breast cancer cells via paracrine down-regulation of MMP expression in fibroblasts and tumour cells. Jones JL, et al. J Pathol. 2003; 201(4):562–72.
- Clinical and functional significance of loss of caveolin-1 expression in breast cancer-associated fibroblasts. Simpkins SA, et al. J Pathol. 2012 Aug;227(4):490-8.
- Adding value to rare tissue samples donated to biobanks characterisation of breast tissue and primary cell cultures obtained from a female-to-male transgender patient. Millican-Slater et al. Cell Tissue Bank 2015; 16:1627–34